Description
This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific for Corticotropin Like Intermediate Lobe Peptide (CLIP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between Horseradish Peroxidase (HRP) labeled Corticotropin Like Intermediate Lobe Peptide (CLIP) and unlabeled Corticotropin Like Intermediate Lobe Peptide (CLIP) (Standards or samples) with the pre-coated antibody specific for Corticotropin Like Intermediate Lobe Peptide (CLIP). After incubation the unbound conjugate is washed off. The amount of bound HRP conjugate is reverse proportional to the concentration of Corticotropin Like Intermediate Lobe Peptide (CLIP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Corticotropin Like Intermediate Lobe Peptide (CLIP) in the sample